The CJC-1295 & GHRP-6 blend have been posited by researchers to display a synergistic action on cells that participate in the release of growth hormone. More specifically, the mix includes CJC-1295, which appears to be similar in action to growth hormone-releasing hormone (GHRH), and GHRP-6 (Growth Hormone Releasing Peptide 6), which appears to bind to the receptors of the hunger hormone ghrelin.This combination of peptides may possibly increase both the strength and the number of growth hormone signals from somatotroph cells. CJC-1295 appears to target the GHRH receptor pathway, while GHRP-6, is considered by researchers to act on the ghrelin receptor pathway. This may explain why the combination of CJC-1295 and GHRP-6 exhibits potential to interact with somatotroph cells in the pituitary gland and the hypothalamus. Both of these regions in the central nervous system are considered to be comprised of cells that are believed to be important in making and controlling growth hormone synthesis.
MOLECULAR FORMULA: C152H252N44O42
MOLECULAR WEIGHT: 3647.954 g/mol
SEQUENCE: H-Tyr-D-Ala-Asp-Ala-Ile-Phe-Thr-Gln-Ser-Tyr-Arg-Lys-Val-Leu-Ala-Gln-Leu-Ser-Ala-Arg-Lys-Leu-Leu-Gln-Asp-Ile-Leu-Ser-Arg-Lys(Mal)-NH2
NOTE: DOES NOT CONTAIN DAC
MOLECULAR FORMULA: C46H56N12O6
MOLECULAR WEIGHT: 873.032 g/mol
SEQUENCE: His-D-Trp-Ala-Trp-D-Phe-Lys
CJC-1295 (Mod GRF 1-29), or tetra-substituted GRF (1-29), is a synthetically developed peptide equivalent to the naturally occurring GHRH hormone. It includes the smallest possible chain of amino acids that might connect to the GHRH receptors, and comprises the initial 29 amino acids of GHRH. The primary structural differences between CJC-1295 and its naturally occurring GHRH equivalent lies in 4 substituted amino acids of GHRH’s original 29 amino acids, to possibly enhance the peptide’s resistance to breakdown by the enzyme dipeptidyl peptidase-4. More specifically, the amino acids that have been altered and substituted are the 2nd, 8th, 15th, and 27th amino acids.[1] Further, the researchers commented, “CJC-1295 caused an increase in total pituitary RNA and GH mRNA, suggesting that proliferation of somatotroph cells had occurred, as confirmed by immunohistochemistry images.” CJC-1295 appears to bind to and interact with the receptor of GHRH in the anterior pituitary gland.[2] It may potentially maintain the pulsatile production of growth hormone (GH), improving overall physiological levels.
GRHP-6, on the other hand, is an opioid analog of the peptide Met-enkephalin. Yet, researchers suggest it lacks the opioid activity generally associated with these enkephalins.[3] GHRP-6 is a synthetic hexapeptide that researchers have categorized as a hormone-releasing peptide. It has been suggested to exhibit action by possibly attaching to the ghrelin receptor found on pituitary cells and specific neurons in the hypothalamus. When it binds, GHRP-6 appears to activate what is known as the growth hormone secretagogue receptor (GHS-R1a). Thus, it has been studied concerning its interaction with the growth hormone secretagogue receptor. GHRP-6 appears to act by the molecular mimicry of the hormone ghrelin, potentially causing a stimulation of the growth hormone secretion. Since growth hormone receptors are present in many cells, the impact of GHRP-6 may be widespread.[4]
The attachment of CJC-1295 seems to initiate processes that result in the discharge of growth hormone (GH) from small sacs within somatotroph cells. Scientists suggest that this peptide may potentially enhance the growth hormone production by these cells, thereby resulting in elevation of “GH concentrations by 2- to 10-fold.”[5] Moreover, the increase in growth hormone appears to be sufficiently lasting to cause a increase in some of the main anabolic mediators of the hormone, such as the so-called insulin-like growth factor 1 (IGF-1). Another research paper comments that there may be a 46% increase in mean growth hormone levels and 45% increase in mean IGF-1 levels following the influence of CJC-1295 in animal research models.[6] Research studies on GRHP-6 have yielded findings that examine GRHP-6’s potential positive impact with the context of various malignant conditions. The GRHP-6 peptide has been primarily researched in conjunction with growth hormone deficiency disorders, Multiple Organ Failure (MOF), cachexia or wasting, obesity, and various eating disorders.[7] Moreover, GHRP-6 may also show a preference for CD36 receptors. These CD36 receptors may have various functions, such as possibly playing a part in metabolism, serving as a collector receptor for fats, aiding in absorption and altering immune reactions, and controlling the process of engulfing particles and inflammation. The pathways of CD36 may also potentially be involved in the management of blood vessel formation.[8] However, these hypotheses require further scientific exploration. Due to its apparent cytoprotective properties, GHRP-6 has been advocated in myocardial infarction research studies, as it may potentially reduce scarring in tissues affected by reperfusion injuries. GRHP-6 may have potential in inducing anti-aging functions within the cell cycle, in addition to possible sleep improvement and cognitive function. When studied in combination, these peptides appear to act synergistically to bring about changes by increasing the growth hormone levels. GHRP-6 appears to maintain a baseline growth hormone level, whereas CJC-1295 mainly appears to stimulate a pulsatile secretion of the growth hormone. However, both peptides have been hypothesized to positively induce improvements within sleep cycles. As most of growth and cellular repair occurs during sleep, the peptide blend has been hypothesized to aid in cellular regeneration and repair. It may possibly improve wound healing and nerve tissue protection via sleep quality enhancement.
CJC-1295 & GHRP-6 peptides have also been associated with nervous tissue protection and repair. One study explored the impact of GHRP-6 on the brain’s IGF-1 system in murine models. The actions of GH are generally considered to be mediated through IGF-I, so the study centered primarily on the modulation of the brain’s IGF system. Researchers reported that the introduction of GHRP-6 for one week appears to have led to an increase in IGF-I mRNA levels in the hypothalamus, cerebellum, and hippocampus, but not in the cerebral cortex. This may suggest that GH and GHRP-6 may enhance the expression of IGF-I in specific brain areas. The study also explored the expression of the IGF receptor and IGF-binding protein IGFBP-2, but its findings reported no considerable fluctuation in activity following peptide introduction. However, the phosphorylation of Akt and Bad appeared stimulated in areas where IGF-I was increased. The researchers noted that this finding implied that GH and GHRP-6 may activate phosphatidylinositol kinase intracellular pathways involved in cell survival in response to growth factors. Bad is a pro-apoptotic member of the Bcl-2 protein family, which is considered to play a central role in cell death. Akt is a serine/threonine-specific protein kinase that plays a key role in multiple cellular processes such as glucose metabolism, apoptosis, cell proliferation, transcription, and cell migration. No reported changes were observed in MAPK, a protein kinase-specific to the amino acids serine, threonine, and tyrosine, nor were changes reported in glycogen synthase kinase-3beta. Moreover, the antiapoptotic protein Bcl-2 was increased in the areas where IGF-I appeared increased, with no change in the proapoptotic protein Bax. This could potentially indicate a shift towards cell survival and away from apoptosis. IGFBP-5, also reported to be involved in neuron survival processes, was reported to be increased mainly in the hypothalamus, which might suggest a potential neuroendocrine role.[9]
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